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transblot turbo semi dry blotting apparatus  (Bio-Rad)


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    Structured Review

    Bio-Rad transblot turbo semi dry blotting apparatus
    Transblot Turbo Semi Dry Blotting Apparatus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 13427 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/semi+dry+transblot+apparatus/Trans-Blot+Turbo+Transfer+System/pm41616925-118-6-11
    Average 99 stars, based on 13427 article reviews
    transblot turbo semi dry blotting apparatus - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Membrane:

    Article Title: Intranasal immunization with influenza antigens conjugated with cholera toxin subunit B stimulates broad spectrum immunity against influenza viruses
    Article Snippet: At 24 h after infection, cells were harvested, and lysed in 1 × cell lysis buffer (Cell Signaling Technology, cat#9803). .. Lysed MDCK cell samples in Laemmli sample buffer (without reducing agent) were loaded and separated on 10% SDS-PAGE, then transferred onto nitrocellulose membrane using a semi-dry transblot apparatus (Bio-rad, cat1703940). .. The membrane was blocked in PBS with 1% Tween (PBST) containing 5% non-fat milk for 1 h and incubated with serum from mice immunized with CTB-3 × M2e-HA2 or NP monoclonal antibody (Abcam, cat#ab20343) at 4 °C overnight.

    Article Title: Identification and Characterization of Tyrosylprotein Sulfotransferase from Human Saliva
    Article Snippet: Electrophoresis was performed in a Mighty Small gel apparatus (Hoefer Scientific Instruments, San Francisco, CA) at 4°C at a constant current of 20 mA per gel for approximately 1 h. Protein bands on the gels were visualized by silver staining. .. Proteins were resolved by SDS-PAGE on a 10% polyacrylamide gel and electrophoretically transferred (20 V for 20 min) to a nitrocellulose membrane (Millipore) by means of a Bio-Rad Semi Dry Transblot apparatus according to the procedure supplied by the Bio-Rad. ..

    Article Title: Live attenuated viral vaccine created by self-attenuation with species-specific artificial MicroRNA
    Article Snippet: .. Lysed MDCK cell samples were loaded and separated on 10% SDS-PAGE, then transferred onto nitrocellulose membrane using a semi-dry transblot apparatus (Biorad, cat1703940). .. The membrane was blocked in PBS with 1% Tween (PBST) and 5% non-fat milk for 1 hour with an anti-NP monoclonal antibody (Abcam, cat# ab20343) at 4° C. overnight.

    Saline:

    Article Title: Natriuretic Peptide Receptor-C Attenuates Hypertension in Spontaneously Hypertensive Rats
    Article Snippet: .. After SDS-PAGE, the separated proteins were electrophoretically transferred to nitrocellulose paper (Schleicher and Schuell Inc., Keene, N.H.) with a semi-dry transblot apparatus (Bio-Rad Laboratories, Mississauga, Ontario) at 15 V for 45 min. After transfer, the membranes were washed twice in phosphate-buffered saline (PBS) and were incubated in PBS containing 8% dehydrated milk at room temperature for 2h. ..

    Article Title: Activation of natriuretic peptide receptor-C attenuates the enhanced oxidative stress in vascular smooth muscle cells from spontaneously hypertensive rats: implication of Gialpha protein.
    Article Snippet: Wehave recently shown that vascular smoothmuscle cells (VSMC) from spontaneously hypertensive rats (SHR) exhibit enhanced expression ofGiα proteins, which was attributed to the enhanced oxidative stress.. Since C-ANP4–23 that specifically interacts with natriuretic peptide C (NPR-C) receptor has been shown to decrease the expression of Giα protein in VSMC, the present study was undertaken to examine if C-ANP4–23 can also decrease the enhanced expression of Giα protein in VSMC from SHR and whether it is attributed to its ability to attenuate the enhanced oxidative stress.. Aortic VSMC from 12-week-old SHR and their age-matched Wistar–Kyoto (WKY) rats were used for the present studies.

    Article Title: Nitric oxide attenuates overexpression of Giα proteins in vascular smooth muscle cells from SHR: Role of ROS and ROS-mediated signaling
    Article Snippet: .. Following separation on SDS-PAGE, proteins were electrophoretically transferred to nitrocellulose paper with a semi-dry transblot apparatus (Bio-Rad Laboratories, Mississauga, Ontario) at 15 V for 45 min. After transfer, the membranes were washed twice in phosphate-buffered saline (PBS) and were incubated in 5% dehydrated milk (w/v in PBS) at room temperature for one hour. .. The blots were washed with PBS containing 0.1% Tween-20 (PBST) and then incubated with respective primary antibodies against AT1(sc-1173), Giα-2 (sc-13534), Giα-3 (sc-262), Nox4 (sc-21860), p47 phox (sc-17845), p22 phox (sc-11712), PDGFR (sc-432), p-PDGFR (sc-12907) EGFR (sc-03), p-EGFR (sc-12351) using different dilutions ranging from 1:500 to 1:2000 at room temperature for 2 h. The antigen-antibody complexes were detected by incubating the blots with goat anti-rabbit IgG (Bio-Rad) conjugated with horseradish peroxidase for 1 h at room temperature.

    Incubation:

    Article Title: Natriuretic Peptide Receptor-C Attenuates Hypertension in Spontaneously Hypertensive Rats
    Article Snippet: .. After SDS-PAGE, the separated proteins were electrophoretically transferred to nitrocellulose paper (Schleicher and Schuell Inc., Keene, N.H.) with a semi-dry transblot apparatus (Bio-Rad Laboratories, Mississauga, Ontario) at 15 V for 45 min. After transfer, the membranes were washed twice in phosphate-buffered saline (PBS) and were incubated in PBS containing 8% dehydrated milk at room temperature for 2h. ..

    Article Title: Activation of natriuretic peptide receptor-C attenuates the enhanced oxidative stress in vascular smooth muscle cells from spontaneously hypertensive rats: implication of Gialpha protein.
    Article Snippet: Wehave recently shown that vascular smoothmuscle cells (VSMC) from spontaneously hypertensive rats (SHR) exhibit enhanced expression ofGiα proteins, which was attributed to the enhanced oxidative stress.. Since C-ANP4–23 that specifically interacts with natriuretic peptide C (NPR-C) receptor has been shown to decrease the expression of Giα protein in VSMC, the present study was undertaken to examine if C-ANP4–23 can also decrease the enhanced expression of Giα protein in VSMC from SHR and whether it is attributed to its ability to attenuate the enhanced oxidative stress.. Aortic VSMC from 12-week-old SHR and their age-matched Wistar–Kyoto (WKY) rats were used for the present studies.

    Article Title: Targeting CRM1 for Progeria Syndrome Therapy
    Article Snippet: The intracellular fluorescence intensity was measured using Image J software, and the data were plotted using GraphPad Prism 8. .. Fibroblast lysates were subjected to electrophoresis on 12% SDS-polyacrylamide gels and transferred onto nitrocellulose membranes (Bio-Rad Laboratories, Hercules CA, USA) using a semi-dry transblot apparatus (Bio-Rad Laboratories) for 1 h. The membranes were blocked in TBST [100 mM Tris-HCl pH 8.0, 150 mM NaCl, 0.5% (v/v) Tween-20] with 5% low-fat dried milk and incubated at 4oC overnight with the proper primary antibodies and the protein signal was developed using horseradish peroxidase-conjugate secondary antibodies (Sigma-Aldrich, MO, USA) and the enhanced chemiluminescence Western blotting detection system (ECLTM; Amersham Pharmacia, GE Healthcare), according to the manufacturer ́s instructions. .. Blot images were acquired using C-Digit® Blot Scanner (LI-COR Biosciences, Lincoln, Nebraska USA).

    Article Title: Nitric oxide attenuates overexpression of Giα proteins in vascular smooth muscle cells from SHR: Role of ROS and ROS-mediated signaling
    Article Snippet: .. Following separation on SDS-PAGE, proteins were electrophoretically transferred to nitrocellulose paper with a semi-dry transblot apparatus (Bio-Rad Laboratories, Mississauga, Ontario) at 15 V for 45 min. After transfer, the membranes were washed twice in phosphate-buffered saline (PBS) and were incubated in 5% dehydrated milk (w/v in PBS) at room temperature for one hour. .. The blots were washed with PBS containing 0.1% Tween-20 (PBST) and then incubated with respective primary antibodies against AT1(sc-1173), Giα-2 (sc-13534), Giα-3 (sc-262), Nox4 (sc-21860), p47 phox (sc-17845), p22 phox (sc-11712), PDGFR (sc-432), p-PDGFR (sc-12907) EGFR (sc-03), p-EGFR (sc-12351) using different dilutions ranging from 1:500 to 1:2000 at room temperature for 2 h. The antigen-antibody complexes were detected by incubating the blots with goat anti-rabbit IgG (Bio-Rad) conjugated with horseradish peroxidase for 1 h at room temperature.

    Western Blot:

    Article Title: Activation of natriuretic peptide receptor-C attenuates the enhanced oxidative stress in vascular smooth muscle cells from spontaneously hypertensive rats: implication of Gialpha protein.
    Article Snippet: Wehave recently shown that vascular smoothmuscle cells (VSMC) from spontaneously hypertensive rats (SHR) exhibit enhanced expression ofGiα proteins, which was attributed to the enhanced oxidative stress.. Since C-ANP4–23 that specifically interacts with natriuretic peptide C (NPR-C) receptor has been shown to decrease the expression of Giα protein in VSMC, the present study was undertaken to examine if C-ANP4–23 can also decrease the enhanced expression of Giα protein in VSMC from SHR and whether it is attributed to its ability to attenuate the enhanced oxidative stress.. Aortic VSMC from 12-week-old SHR and their age-matched Wistar–Kyoto (WKY) rats were used for the present studies.

    Article Title: Targeting CRM1 for Progeria Syndrome Therapy
    Article Snippet: The intracellular fluorescence intensity was measured using Image J software, and the data were plotted using GraphPad Prism 8. .. Fibroblast lysates were subjected to electrophoresis on 12% SDS-polyacrylamide gels and transferred onto nitrocellulose membranes (Bio-Rad Laboratories, Hercules CA, USA) using a semi-dry transblot apparatus (Bio-Rad Laboratories) for 1 h. The membranes were blocked in TBST [100 mM Tris-HCl pH 8.0, 150 mM NaCl, 0.5% (v/v) Tween-20] with 5% low-fat dried milk and incubated at 4oC overnight with the proper primary antibodies and the protein signal was developed using horseradish peroxidase-conjugate secondary antibodies (Sigma-Aldrich, MO, USA) and the enhanced chemiluminescence Western blotting detection system (ECLTM; Amersham Pharmacia, GE Healthcare), according to the manufacturer ́s instructions. .. Blot images were acquired using C-Digit® Blot Scanner (LI-COR Biosciences, Lincoln, Nebraska USA).

    SDS Page:

    Article Title: Identification and Characterization of Tyrosylprotein Sulfotransferase from Human Saliva
    Article Snippet: Electrophoresis was performed in a Mighty Small gel apparatus (Hoefer Scientific Instruments, San Francisco, CA) at 4°C at a constant current of 20 mA per gel for approximately 1 h. Protein bands on the gels were visualized by silver staining. .. Proteins were resolved by SDS-PAGE on a 10% polyacrylamide gel and electrophoretically transferred (20 V for 20 min) to a nitrocellulose membrane (Millipore) by means of a Bio-Rad Semi Dry Transblot apparatus according to the procedure supplied by the Bio-Rad. ..

    Electrophoresis:

    Article Title: Targeting CRM1 for Progeria Syndrome Therapy
    Article Snippet: The intracellular fluorescence intensity was measured using Image J software, and the data were plotted using GraphPad Prism 8. .. Fibroblast lysates were subjected to electrophoresis on 12% SDS-polyacrylamide gels and transferred onto nitrocellulose membranes (Bio-Rad Laboratories, Hercules CA, USA) using a semi-dry transblot apparatus (Bio-Rad Laboratories) for 1 h. The membranes were blocked in TBST [100 mM Tris-HCl pH 8.0, 150 mM NaCl, 0.5% (v/v) Tween-20] with 5% low-fat dried milk and incubated at 4oC overnight with the proper primary antibodies and the protein signal was developed using horseradish peroxidase-conjugate secondary antibodies (Sigma-Aldrich, MO, USA) and the enhanced chemiluminescence Western blotting detection system (ECLTM; Amersham Pharmacia, GE Healthcare), according to the manufacturer ́s instructions. .. Blot images were acquired using C-Digit® Blot Scanner (LI-COR Biosciences, Lincoln, Nebraska USA).



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